TRANSPLANTABILITY OF HUMAN LYMPHOID-CELL LINE, LYMPHOMA, AND LEUKEMIA IN SPLENECTOMIZED AND-OR IRRADIATED NUDE-MICE

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (7) , 2588-2595
Abstract
The effects of splenectomy and/or whole-body irradiation of nude mice before xenotransplantation of lymphoid cell lines, lymphoma and leukemia were studied. Transplantation after whole-body irradiation increased the take rate of 3 cultured cell lines (2 of T[thymus-derived]-cell-derived acute lymphocytic leukemia and 1 of B[bone marrow-derived]-cell-derived acute lymphocytic leukemia) and in the tumorous growth of Burkitt-derived Raji and spontaneously transformed lymphoblastoid cell lines. With splenectomy plus irradiation pretreatment, tumorous growth occurred in 4 other cell lines which were not transplantable after irradiation only (2 cell lines of Epstein-Barr virus-transformed cord blood cells and 1 each of null acute lymphocytic leukemia and nodular lymphoma-derived cell lines). Direct transplantation of leukemia and lymphoma cells into the pretreated mice was successful in 7 of 24 cases (29%). B-cell-derived diffuse large lymphoid lymphoma was transplantable in 3 of 7 cases (43%). Lymphoma and leukemia of peripheral T-cell origin were difficult to transplant even with pretreatment, and only 1 pleomorphic T-cell lymphoma grew to a significant size (2 cm). One tumor each of B-cell-derived diffuse large lymphoid lymphoma and T-cell diffuse lymphoblastic lymphoma became transplantable.