Cultivation ofLeishmania: comparison of different media for promastigote cultivation
- 1 December 1982
- journal article
- research article
- Published by Taylor & Francis in Pathogens and Global Health
- Vol. 76 (6) , 607-613
- https://doi.org/10.1080/00034983.1982.11687590
Abstract
Experiences in the cultivation of promastigotes of Leishmania both for maintenance in the laboratory and for growth in mass culture were summarized. Maintenance studies were carried out with a L. donovani strain which was isolated in Murcia [Spain] and cultivated in the following media: NNN, NNN with Hank''s solution overlay, NNN with brain-heart [from rabbit] infusion overlay, Mathis''s medium, Weinman''s medium, brain-heart Infusion blood, semi-solid brain-heart infusion medium, brain-heart infusion haemin medium, brain-heart infusion/lysed blood and NNN condensation liquid. For mass cultivation LV 9 (L. donovani) and LV 160 (L. mexicana amazonensis) strains from the Liverpool [UK] collection were used; these were cultivated in the following media: brain-heart infusion/lysed blood, Schneider''s, Homen''s and brain-heart infusion media.This publication has 15 references indexed in Scilit:
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