Long-Term Protection against Tuberculosis following Vaccination with a Severely Attenuated Double Lysine and Pantothenate Auxotroph ofMycobacterium tuberculosis

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Abstract
We report the safety and immunogenicity of a double lysine and pantothenate auxotroph ofMycobacterium tuberculosisin mice. The ΔlysAΔpanCDmutant is completely attenuated in immunocompromised SCID and gamma interferon knockout mice yet induces short-term and long-term protection in immunocompetent and CD4-deficient mice following single-dose subcutaneous vaccination.