Development of a flow injection immunoanalysis (FIIA) for pesticide determination by means of fluorescence detection

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Abstract
In this paper we describe the state of development of a flow-through immunosensor for quasi- continuous monitoring of pesticides, especially triazine herbicides, in drinking water. Starting with time-resolved fluoroimmunoassays using Eu-chelates as fluorescent labels, we obtained a detection limit of 0.1 (mu) g/l for some triazine herbicides. The experimental parameters applied in assays on microtiter strips were transferred and adjusted to small carrier beads as solid phase in affinity columns. For fluorescence detection in the FIIA a pulsed laser was used as an excitation source. The light was coupled into a flow-through cuvette and the fluorescence signal was recorded with a photomultiplier tube working in a gated detection mode.

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