Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b
- 1 February 1991
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 5 (2) , 335-342
- https://doi.org/10.1111/j.1365-2958.1991.tb02114.x
Abstract
The oxidation of methane to methanol in methano-trophic bacteria is catalysed by the enzyme methane monooxygenase (MMO). This multicomponent enzyme catalyses a range of oxidations including that of aliphatic and aromatic compounds and therefore has potential for commercial exploitation. This study details the molecular characterization of the soluble MMO (sMMO) genes from the Type II methanotroph Methylosinus trichosporium OB3b. The structural genes encoding the α, β and γ subunits of sMMO protein A and the structural gene encoding component B have been isolated and sequenced. These genes have been expressed and their products identified using an in vitro system. A comparative analysis of sMMO predicted sequences of M. trichosporium OB3b and the taxonomically related M. capsulatus (Bath) is also presented.Keywords
This publication has 32 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsPublished by Elsevier ,2006
- CLUSTAL: a package for performing multiple sequence alignment on a microcomputerPublished by Elsevier ,2003
- Cloning, sequencing and expression of the glutamine synthetase structural gene (glnA) from the obligate methanotroph Methylococcus capsulatus (Bath)Journal of General Microbiology, 1990
- The methane monooxygenase gene cluster of Methylococcus capsulatus (Bath)Gene, 1990
- Purification of a high specific activity methane monooxygenase hydroxylase component from a type II methanotrophBiochemical and Biophysical Research Communications, 1988
- Restoration of phenotype inEscherichia coliauxotrophs by pULB113-mediated mobilisation from methylotrophic bacteriaFEMS Microbiology Letters, 1987
- Isolation of methane monooxygenase-deficient mutants fromMethylosinus trichosporiumOB3b using dichloromethaneFEMS Microbiology Letters, 1987
- Acetylene as a suicide substrate and active site probe for methane monooxygenase fromMethylococcus capsulatus(Bath)FEMS Microbiology Letters, 1985
- The Effect of Growth Conditions on Intracytoplasmic Membranes and Methane Mono-oxygenase Activities in Methylosinus trichosporium OB3bMicrobiology, 1981
- Mutation in the obligate methylotrophsMethylococcus capsulatusandMethylomonas albusFEMS Microbiology Letters, 1977