Sequence analysis of cfxA2-like beta-lactamases in Prevotella species
Open Access
- 14 April 2003
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 51 (5) , 1293-1296
- https://doi.org/10.1093/jac/dkg221
Abstract
Sixty-two strains of oral (32) and non-oral (30) Prevotella producing β-lactamases were screened for cfxA by PCR, using an intragenic primer pair. All 62 were cfxA/cfxA2 positive. Fourteen of these strains, representing seven pigmented and seven non-pigmented Prevotella species were submitted to further PCR with specific primers that amplified the whole β-lactamase structural gene (966 bp). After cloning and sequencing, the deduced amino acid sequences were compared with that of Bacteroides vulgatus CfxA β-lactamase. All 14 sequences possessed the E272K substitution characteristic of CfxA2. CfxA sensu stricto was not observed in the present series. G83D, F/V189L, W193L and D239Y substitutions were observed more than once, without species specificity. This sequence analysis indicates that most oral and non-oral β-lactamase-producing Prevotella isolates from French patients produce variants of the CfxA enzyme.Keywords
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