Rescue and propagation of fully retargeted oncolytic measles viruses
Top Cited Papers
- 30 January 2005
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 23 (2) , 209-214
- https://doi.org/10.1038/nbt1060
Abstract
Live attenuated measles viruses of the Edmonston lineage (MV-Edm) have potent anti-tumor activity1,2,3,4 but are not entirely tumor-specific owing to widespread distribution of their native receptors, CD465,6 and SLAM7,8,9. We have therefore developed a pseudoreceptor system that allows rescue and propagation of fully retargeted viruses displaying single-chain antibody fragments. Viruses retargeted to tumor-selective CD38, epidermal growth factor receptor (EGFR) or EGFR mutant vIII (EGFRvIII) efficiently entered cells through their respective targeted receptors in vitro and in vivo, but not through CD46 and SLAM. When administered intratumorally or intravenously to mice bearing human CD38 or EGFR-positive human tumor xenografts, the targeted viruses demonstrated specific receptor-mediated anti-tumor activity. These data provide an in vivo demonstration of antibody-directed tumor destruction by retargeted oncolytic viruses.Keywords
This publication has 36 references indexed in Scilit:
- Targeting virus entry and membrane fusion through specific peptide/MHC complexes using a high-affinity T-cell receptorGene Therapy, 2004
- Antibody-targeted cell fusionNature Biotechnology, 2004
- Biodistribution of Oncolytic Measles Virus After Intraperitoneal Administration into Ifnar™-CD46Ge Transgenic MiceHuman Gene Therapy, 2003
- CD46- and CD150-independent endothelial cell infection with wild-type measles virusesJournal of General Virology, 2003
- Measles Virus Infects and Suppresses Proliferation of T Lymphocytes from Transgenic Mice Bearing Human Signaling Lymphocytic Activation MoleculeJournal of Virology, 2003
- Identification of a Second Major Site for CD46 Binding in the Hemagglutinin Protein from a Laboratory Strain of Measles Virus (MV): Potential Consequences for Wild-Type MV InfectionJournal of Virology, 2002
- SLAM (CD150)-Independent Measles Virus Entry as Revealed by Recombinant Virus Expressing Green Fluorescent ProteinJournal of Virology, 2002
- Crystal Structure of the Anti-His Tag Antibody 3D5 Single-chain Fragment Complexed to its AntigenJournal of Molecular Biology, 2002
- Single-Chain Antibody Displayed on a Recombinant Measles Virus Confers Entry through the Tumor-Associated Carcinoembryonic AntigenJournal of Virology, 2001
- The human CD46 molecule is a receptor for measles virus (Edmonston strain)Cell, 1993