Electrophoretic separation of proteins from roots and root exudates

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Abstract
The total protein concentration of exudates released from sterile intact roots of various species (e.g., corn, pea, sunflower) fluctuated markedly during plant development. One major, sometimes with two or three minor, fast-moving soluble protein bands (globulin and albumin) and one weak basic protein band were detected by disc electrophoresis at various developmental stages, whereas 12 to 14 soluble protein bands were detected in seedling roots and at least 16 were distinguished in mature roots. Only one basic protein band was detected in root homogenates and exudates. When individual exudate samples collected at about weekly intervals for 85 days were combined before analysis, 9 to 14 bands were detected, indicating that many, but not all, of the proteins present in roots were liberated, but, with the exception of one or two components, in quantities too low for detection. Comparison of exudates from plants grown in sand or liquid culture indicated that release of proteins did not result from injury of the roots by the sand particles.

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