Sequence‐dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H
Open Access
- 11 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 215 (2) , 327-330
- https://doi.org/10.1016/0014-5793(87)80171-0
Abstract
We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo‐ and 2′‐O‐methylnucleosides, a nonaribonucleotide, [5′‐32 pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3′m(UG)d(AATG)m(GAC)5′ was used as a hybridization strand, *pACUUACCUG was cleaved between C6 and C7 to yield *pACUUAC. In the presence of 3′m(UGAA)d(TGGA)m(C)5′, the nonaribonucleotide was hydrolyzed, mainly between U8 and C9, to give *pACUUACCU. This method will have a variety of applications in the field of RNA engineering.Keywords
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