Two‐dimensional electrophoresis of plant proteins and standardization of gel patterns
- 1 January 1996
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 17 (5) , 855-865
- https://doi.org/10.1002/elps.1150170507
Abstract
Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two‐dimensional electrophoresis analysis with two modifications: (i) comigration of external standard marker proteins with resultant horizontal and vertical stripes in the gel, and (ii) deblocking with a vapor of aqueous heptafluorobutyric acid for N‐acetylserine. Approximately 5000 protein spots were separated from both the five tissues of Arabidopsis and the nine tissues of rice. Over one hundred spots were electroblotted for N‐terminal sequencing. Among the newly sequenced proteins, 62 were from Arabidopsis and 51 from rice.Keywords
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