Antibacterial Activity of Lactoperoxidase Adsorbed by Human Salivary Sediment and Hydroxyapatite

Abstract
Lactoperoxidase was shown to be adsorbed by human salivary sediment and hydroxyapatite. Phosphate ions (0.5 M) effectively regained the adsorbed activity while sodium fluoride (1 mg/l) was less effective. 45% of the total activity was adsorbed by salivary sediment in whole saliva solution and 64% in distilled water. In case of hydroxyapatite the respective values were 97 and 93%. The adsorbed peroxidase was shown to be antibacterial in the presence of cofactors, SCN–– ions and H2O2. The complete lactoperoxidase system inhibited remarkably the acid production of dental plaque when compared with the control.