The Nutrition of Protozoa

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Abstract
The principle upon which this Method was based was control of the proportions of alcohol and eTher used during the fractionation process so that a maximum extraction of serum lipids could be effected without precipitating the residual serum proteins. Two fractions were obtained: (1) a lipid fraction which was shown to be free from protein and (2) a protein fraction which contained all of the proteins in serum precipitable by 50% alcohol. Both of these fractions were necessary in order to obtain growth of the organism in numbers equivalent to that obtained with intact serum. Several advantages are evident in the procedure outlined. Relatively large quantities of serum may be fractionated at room temps. with simple labora- tory apparatus. The fractions obtained pass readily through bacteria-retaining sterile filters and possess a high degree of activity. Separation of the fractions from the standpoint of the T. vaginalis assay is excellent.