Response of Solid Tumor Cells Exposed to Chemotherapeutic Agents In Vivo: Cell Survival After 2- and 24-Hour Exposure2

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Abstract
The cytotoxic effects of single, graded doses of 3 chemotherapeutic agents (cyclophosphamide, 5-fluorouracil, and bleomycin), given to mice bearing the EMT6 mammary sarcoma were measured by determining, in vitro, the clonogenic capacity of the tumor cells. Animals were killed 2 or 24 hours after drug administration. Survival curves of cells from both 2- and 24-hour tumors were biphasic, possibly indicating differential responses of the cycling and the noncycling cell compartments. However, plating efficiencies of cells from 24-hour tumors were consistently and substantially higher than those from 2-hour tumors. Repair of some potentially lethal lesions in the 22-hour interval most satisfactorily explains the increase. Some implications of the findings for chemotherapy are discussed.

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