Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers
- 1 January 2007
- book chapter
- Published by Springer Nature
- Vol. 400, 515-529
- https://doi.org/10.1007/978-1-59745-519-0_35
Abstract
Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from γ and CHIF, two membrane proteins that function as regulatory subunits of the Na+- and K+-ATPase.Keywords
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