A simple method for purification of rat hepatic microsomal cytochrome b5
- 15 February 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 226 (1) , 339-341
- https://doi.org/10.1042/bj2260339
Abstract
Rat hepatic microsomal cytochrome b5 was purified to homogeneity by solubilization with the detergent Lubrol 12-A9 and chromatography on Fractogel TSK DEAE-650(S). The protein was obtained in high yield (52-87%) in 8 h, and only 1 polypeptide band, of MW 16,600, was visible after sodium dodecyl sulfate/polyacrylamide-gel electrophoresis.This publication has 20 references indexed in Scilit:
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