High-Performance Liquid Chromatographic Determination of L-Aspartyl-L-Phenylalanine Methyl Ester in Various Food Products and Formulations

Abstract
A simple, rapid, and specific high-performance liquid chromatographic (HPLC) procedure is described for the analysis of the chemical sweetener L-aspartyl-L-phenylalanine methyl ester (aspartame). Using a strong cation exchange column and pressures <1000 psig, an analysis can be performed in <15 min. The technique has been applied to a wide range of food products and formulations. No interferences were found in the samples studied. Recoveries are quantitative, and the coefficients of variation for replicate analyses are ≤2.5%.

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