An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the AutoAnalyzer
- 1 September 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 11 (9) , 876-888
- https://doi.org/10.1093/clinchem/11.9.876
Abstract
A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple manifold design. Correlations are presented between the manual procedure and the automated system.Keywords
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