Abstract
Egg-grown chlamydiae (EGO) have a yolk sac antigen associated with their surface which is absent from cell monolayer-grown organisms (CGO). EGO infectivity was specifically neutralized by rabbit antiserum to normal yolk sac; CGO infectivity before or after incubation with normal yolk sac material was not neutralized. Treatment of EGO with Clostridium welchii [C. perfringens] culture filtrate containing phospholipase C abolished spontaneous infectivity for monolayers and neutralization by anti-yolk sac antiserum but did not affect centrifuge-assisted infectivity. The possible significance of host antigen on the chlamydial surface is considered.

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