Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light
Open Access
- 1 November 1972
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11) , 3459-3463
- https://doi.org/10.1073/pnas.69.11.3459
Abstract
Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.Keywords
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