An Electron Microscope Study of Chorioallantoic Membrane Infected with the Virus of Avian Infectious Bronchitis

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Abstract
Ultra-thin sections of chick chorioallantoic membranes infected with avian infectious bronchitis virus were examined in an RCA EMU2A electron microscope. Nearly all the particles of the avian infectious bronchitis virus were electron dense spherical bodies averaging 178 mu in diameter. The virus particles were arranged in pairs, singly, in chains, and in clumps. Variations in electron density within certain individual particles gave them a doughnut appearance. Some virus particles exhibited a discrete internal structure made up of rows of clear spots of less electron density, which contained a smaller electron dense body. No normal tissue was found in infected tissue sections.