A Solid Matrix Bioassay for Determining Chemical Feeding Stimulants

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Abstract
Attempts to manipulate the chemical composition of fish feeds and baits to increase their efficacy have required experimental studies to determine feeding preferences for species of interest. This article describes a generally useful assay method in which test stimuli are incorporated into a solid agar matrix. Agar disks are then presented to fish in a two-choice feeding assay. The agar disk assay is simple and inexpensive, requires only small quantities of test materials, and gives direct information about consumption and preference.