Biosynthesis of oleanene- and ursene-type triterpenes from [4-13C]mevalonic acid in tissue cultures of Isodon japonicus Hara

Abstract
On the basis of the 13C-labelling patterns elucidated by 13C n.m.r. spectroscopy in oleanene- and ursene-type triterpenes (1)–(5) isolated from Isodon japonicus tissue cultures fed with [4-13C]mevalonic acid, Ruzicka's hypothesis for cyclisation of squalene 2,3-oxide to the pentacyclic triterpenes has been verified experi-mentally.

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