A Fluorometric Enzyme Inhibition Detector for Carbamate Pesticide Analysis by High Speed Liquid Chromatography

Abstract
A fluorometric enzyme inhibition detector has been developed for the detection of carbamate pesticides after separation using high speed liquid chromatography. Housefly head cholinesterase was found to have the greatest sensitivity toward the carbamates, together with horse plasma and bovine erythrocyte cholinesterases. The substrate N-methyl indoxyl acetate is used for monitoring of enzyme activity.