The La antigen shuttles between the nucleus and the cytoplasm in CV-1 cells
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 85 (2) , 103-114
- https://doi.org/10.1007/bf00577106
Abstract
Recently we established a monoclonal antibody against the La-protein (Bachmannet al., Proc. Natl. Acad. Sci. USA, 83, 7770, 1986). The antibody gives a nuclear speckled type staining and, in addition, a perinuclear cytoplasmic staining on cultured cells in immunofluorescence microscopy. After inhibition of RNA synthesis the La-protein is transported into the cytoplasm. After prolonged inhibition it returns into the nucleus forming large growing speckles. The transport into the nucleus apparently depends on glycosylation.Keywords
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