EVENTS IN THE PHYTOCHROME MOLECULE AFTER IRRADIATION
- 1 November 1992
- journal article
- review article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 56 (5) , 803-809
- https://doi.org/10.1111/j.1751-1097.1992.tb02236.x
Abstract
The photoconversion of Pr to Pfr has been investigated by a large number of investigators. We have previously demonstrated that Z, E isomerization of the tetrapyrrole chromophore is involved in the photoconversion. It is the best candidate for the primary photoreaction. Conformation and configuration of the Pr chromophore will be compared with that of chromophores in phycocyanin. The crystal structure of phycocyanin had been elucidated by x-ray analysis. Proton transfer and/or Z, E isomerization of the tetrapyrrole are probably involved in different steps of the photoconversion in phytochrome and in photoreversible phycobiliproteins. Fluorescence decay kinetics of irradiated Pr and intermediate formation show heterogeneity. Possible reasons for this heterogeneity will be discussed.Keywords
This publication has 63 references indexed in Scilit:
- Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66 Å resolutionJournal of Molecular Biology, 1991
- THE PHOTOTRANSFORMATION PATHWAY OF DIMERIC OAT PHYTOCHROME FROM THE RED‐LIGHT‐ABSORBING FORM TO THE FAR‐RED‐LIGHT‐ABSORBING FORM AT PHYSIOLOGICAL TEMPERATURE IS COMPOSED OF FOUR INTERMEDIATESPhotochemistry and Photobiology, 1990
- Fluorescence studies of phytochrome in the cells of etiolated oat seedlings and 124-kDa phytochrome isolated from themJournal of Photochemistry and Photobiology B: Biology, 1990
- Refined three-dimensional structure of phycoerythrocyanin from the cyanobacterium Mastigocladus laminosus at 2.7 ÅJournal of Molecular Biology, 1990
- Excited complexes in triethylamine—ethanol at low temperatures: pulse radiolysis studyJournal of Photochemistry and Photobiology A: Chemistry, 1989
- Primary photoprocesses of phytochrome. Picosecond fluorescence kinetics of oat and pea phytochromesBiochemistry, 1989
- Molecular characterization and evolution of sequences encoding light-harvesting components in the chromatically adapting cyanobacterium Fremyella diplosiphonJournal of Molecular Biology, 1988
- Heterogeneity of the amino acid sequence of phytochrome from etiolated oat seedlingsFEBS Letters, 1987
- Refined three-dimensional structures of two cyanobacterial C-phycocyanins at 2.1 and 2.5 Å resolutionJournal of Molecular Biology, 1987
- Crystal structure analysis and refinement at 2·5 Å of hexameric C-phycocyanin from the cyanobacterium Agmenellum quadruplicatumJournal of Molecular Biology, 1986