Serum‐free culture of hematopoietic stem cells: A Review
- 1 January 1995
- journal article
- review article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 13 (6) , 607-612
- https://doi.org/10.1002/stem.5530130605
Abstract
The development of serum‐free systems for the maintenance and expansion of both primitive and committed hematopoietic progenitors has numerous applications in both basic and clinical research. Many different media have been tested and refined over the years, and current formulations now yield results similar to those observed with fetal bovine serum‐based medias. Using these serum‐free culture systems, the impact of the cell microenvironment and individual growth factors on primitive and maturing stem cells have both been studied. The utility of progenitor populations expanded ex vivo under serum‐free conditions is under investigation.Keywords
This publication has 26 references indexed in Scilit:
- In vitro growth of human fetal CD34+cells in the presence of various combinations of recombinant cytokines under serum-free culture conditionsBritish Journal of Haematology, 1994
- Lineage commitment in human hemopoiesis involves asymmetric cell division of multipotent progenitors and does not appear to be influenced by cytokinesJournal of Cellular Physiology, 1993
- Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factorsCell, 1993
- Ontogeny-related changes in proliferative potential of human hematopoietic cells.The Journal of Experimental Medicine, 1993
- Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.The Journal of Experimental Medicine, 1992
- Serum-free culture of human hemopoietic progenitors in attenuated culture mediaAmerican Journal of Hematology, 1988
- RECONSTITUTION OF HAEMOPOIETIC SYSTEM WITH AUTOLOGOUS MARROW TAKEN DURING RELAPSE OF ACUTE MYELOBLASTIC LEUKAEMIA AND GROWN IN LONG-TERM CULTUREThe Lancet, 1986
- Serum‐free culture of primitive murine hemopoietic colony‐forming cellsThe International Journal of Cell Cloning, 1986
- Complete replacement of serum in cultures of murine primitive erythroid and multipotential progenitor cells: absolute requirement for spleen conditioned mediumCell Differentiation, 1985
- Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell culturesNature, 1976