Location, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.
- 1 January 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (1) , 147-151
- https://doi.org/10.1073/pnas.78.1.147
Abstract
The major promoters for bacteriophage T3 RNA polymerase on the T3 genome were mapped by DNA-RNA filter hybridization. One promoter is located in a 300 base pair Hpa I restriction fragment near the genetic left end of T3 DNA. The sequence in the vicinity of the major initiation site of transcription in this region was determined. A part of the (-)strand sequence is .**GRAPHIC**. Comparison of this sequence with the prototype 23 base pair promoter sequence for bacteriophage T7 RNA polymerase shows a striking pattern of homology and divergence. Between positions -9 and +4, the sequences are virtually identical; between positions -17 and -10, the sequences are quite different. These sequence subsets may perform different functions in transcription initiation by the phage RNA polymerases.This publication has 25 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.Proceedings of the National Academy of Sciences, 1980
- Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequenceNature, 1979
- Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.Proceedings of the National Academy of Sciences, 1975
- Fidelity of in Vitro Transcription of T3 Deoxyribonucleic Acid by Bacteriophage T3-induced Ribonucleic Acid Polymerase and by Escherichia coli Ribonucleic Acid PolymeraseJournal of Biological Chemistry, 1974
- Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells.1973
- Transcription in vitro of T3 DNA by Escherichia coli and T3 RNA PolymerasesEuropean Journal of Biochemistry, 1972
- In vitro transcription of T3 DNA by Escherichia coli and T3 polymerasesVirology, 1972
- Initiation, Release, and Reinitiation of RNA Chains by Bacteriophage-T3-Induced Polymerase from T3 DNA TemplatesProceedings of the National Academy of Sciences, 1972
- Induction of a new RNA polymerase in infected with bacteriophage T3Biochemical and Biophysical Research Communications, 1971