A Study of Different Methods for the Preparation of B. Typhosus Antigen

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Abstract
One of the reasons generally assigned for the unsatisfactory status of complement-fixation tests in the diagnosis of bacterial infections, is the difficulty of preparing efficient and stable antigens; among the diseases of bacterial origin complement-fixation is probably most widely employed in the diagnosis of glanders, tuberculosis and gonococcus infections, but in the last mentioned the test is well known as lacking in the sufficient delicacy and it probably can be rendered more sensitive by further improvement of the antigen, and the same may be true of the tuberculosis complement-fixation test. Bacterial antigens for complement-fixation tests may be divided into four main groups, namely (a) those composed of whole bacteria and their soluble products in the fluid medium in which they have been cultivated; (b) those in which the bacterial cells alone are utilized suspended in sterile salt solution; (c) those in which the bacterial cells are disrupted but not filtered and (d) those in which the cells are disrupted and the insoluble portions removed by filtration, the filtrate being employed as antigen.

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