Estimation of daily sperm production rate (DSPR) by quantitative testicular histology in Buffalo-Bulls (Bubalus Bubalis)

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Abstract
To ensure maximum use of good quality males in a breeding program their sperm production rate must be known, because such information is useful in predicting their reproductive capacity. Testes and epididymides from 6 sexually rested buffalo-bulls were removed during breeding season. The average weight of the testicular parenchyma was 138.62 g, of which the tunica albuginea accounted for 8.45 g. Relative distribution of spermatozoa in caput, corpus and cauda epididymides averaged 5.42, 0.75 and 11.45 billion, respectively. Total epididymal sperm reserve per bull was 36.2 billion. The efficiency of sperm production was quite uniform and averaged 14.5 .times. 106 sperm/g of testicular parenchyma per day with a mean of 2.02 .times. 109 sperm/testis. Thus a typical buffalo-bull produces about 4 .times. 109 sperm daily during breeding season.