Cyclic Nucleotide Phosphodiesterase in Dictyostelium discoideum : Interconversion of Two Enzyme Forms
- 3 March 1972
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 175 (4025) , 1016-1018
- https://doi.org/10.1126/science.175.4025.1016
Abstract
An extracellular cyclic nucleotide phosphodiesterase was isolated from either growing cultures or aggregating amoebas of Dictyostelium discoideum . The enzyme is released in a form with a low Michaelis constant (15 micromolar) and spontaneously undergoes a slow conversion to a less active form with a high Michaelis constant (2 millimolar). Inactivation was prevented or reversed by use of Cleland's reagent, dithiothreitol. The two enzyme forms may be part of a mechanism for control of concentration of cyclic adenosine monophosphate.Keywords
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