Identification of a novel nicotinic acetylcholine receptor structural subunit expressed in goldfish retina.
Open Access
- 1 February 1989
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 108 (2) , 637-645
- https://doi.org/10.1083/jcb.108.2.637
Abstract
A new nonalpha (n.alpha.) member of the nicotinic acetylcholine receptor (nAChR) gene family designated GFn.alpha.-2 has been identified in goldfish retina by cDNA cloning. This cDNA clone encodes a protein with structural features common to all nAChR subunits sequenced to date; however, unlike all known .alpha.-subunits of the receptor, it lacks the cysteine residues believed to be involved in acetylcholine binding. Northern blot analysis shows multiple transcripts hybridizing to the GFn.alpha.-2 cDNA in goldfish retina but undetectable levels of hybridizable RNA in brain, muscle, or liver. S1 nuclease protection experiments indicate that multiple mRNAs are expressed in retina with regions identical or very similar to the GFn.alpha.-2 sequence. In situ hybridization shows that the gene encoding GFn.alpha.-2 is expressed predominantly in the ganglion cell layer of the retina.Keywords
This publication has 56 references indexed in Scilit:
- cDNA clones coding for the structural subunit of a chicken brain nicotinic acetylcholine receptorNeuron, 1988
- Rapid expression of novel proteins in goldfish retina following optic nerve crushBiochemical and Biophysical Research Communications, 1986
- Isolation and sequence of cDNA clones coding for the precursor to the γ subunit of mouse muscle nicotinic acetylcholine receptorJournal of Neuroscience Research, 1986
- The functions of acetylcholine in the rabbit retinaProceedings of the Royal Society of London. B. Biological Sciences, 1984
- Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probesDevelopmental Biology, 1984
- Characterization of the α‐Bungarotoxin Receptor in Chick‐Embryo RetinaEuropean Journal of Biochemistry, 1981
- Acetylcholine Synthesis by Displaced Amacrine CellsScience, 1980
- Histological localization of binding sites of α-bungarotoxin and of antibodies specific to acetylcholine receptor in goldfish optic nerve and tectumBrain Research, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Changes in the morphology and amino acid incorporation of regenerating goldfish optic neuronsExperimental Neurology, 1969