SUB-POPULATIONS OF IG-SECRETING CELLS INDUCED BY PEROXIDASE IMMUNIZATION - DISCRIMINATION ACCORDING TO ANTIBODY STORAGE AND SECRETION

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 36  (4) , 719-727
Abstract
Mice were injected in their hind footpads with peroxidase (PO) emulsified in Freund''s complete adjuvant. The development of cells secreting anti-peroxidase antibody (Ab) and cells secreting immunoglobulins (Ig) were detected in the draining popliteal lymph nodes in the subsequent 35 days, using local hemolysis plaque assay with sheep red blood cells coated with PO or anti-mouse Ig Ab. Plaque-forming cells (PFC) were isolated from the center of plaques by micromanipulation and after appropriate treatment were examined by EM for their intracellular Ab content and incorporation of [3H]-thymidine. Four subpopulations of Ig secreting cells were distinguished: cells secreting Ig without Ab function and not containing intracellular Ab detectable between days 5 and 20; cells secreting Ig without Ab function but containing Ab appearing on day 6 and present throughout the immune response; cells secreting Ab and containing Ab; and cells secreting Ab, but without detectable intracellular Ab. These last subpopulations appeared on day 7 and were found in all subsequent assays. Cells secreting Ig without Ab function might be precursors of Ab secreting cells.