Allozyme Markers in the Study of Sperm Precedence in the Plum Curculio, Conotrachelus nenuphar1,2
- 17 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of the Entomological Society of America
- Vol. 69 (3) , 465-468
- https://doi.org/10.1093/aesa/69.3.465
Abstract
Allozymes (genetically polymorphic enzymes) assayed by horizontal starch gel electrophoresis were used as genetic markers in a study of sperm precedence in the plum curculio, Conotrachelus nenuphar (Herbst). Two strains having greatly different frequencies of an isocitrate dehydrogenase allele were crossed. Three other allozyme loci were also assayed in both parents and progeny. Sperm precedence of the type in which sperm from the 2nd mating replace those of the 1st for fertilization was found: 17 of 23 crosses (73.9%) showed sperm precedence. Sperm from 1st matings were used to fertilize eggs after the 2nd matings in 31.2% of the crosses. The mean percentage of progeny produced after the 2nd mating by sperm from the 1st mating was 14.23% . A minimum mean refractory period of 2.2 wk was found in the female before remating would occur.This publication has 1 reference indexed in Scilit:
- Mating Studies with Apholate-Sterilized Boll Weevils1Journal of Economic Entomology, 1967