Preparative, Electroenzymatic Reduction of NAD+ to NADH on a Thin Poly(Acrylic Acid) Layer-Coated Graphite Felt Electrode Coimmobilizing Ion-paired Methyl Viologen-Cation-Exchange Polymer and Diaphorase

Abstract
A thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen – cation-exchange polymer and diaphorase was prepared and applied to preparative electroenzymatic reduction of NAD+ in phosphate buffer at constant potential of −0.80 V vs. SCE. The electrode was stable for the reaction and yielded NADH quantitatively in 97.8% current efficiency.

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