IMPROVEMENT OF THE QUANTITATIVE-DETERMINATION OF VERAPAMIL IN HUMAN-PLASMA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 17  (10) , 375-379
Abstract
An improvement in the extraction procedure for the quantitative determination of verapamil [antiarrhythmic agent] in human plasma is described. The advantages are: the time involved in the extraction procedure is only 1/3 of that in the original method allowing the extraction of 100 samples in a working day; increased mean recovery from 67-85% over the range of 3-90 ng verapamil/ml plasma; and less expenditure. The lower limit of detection is 3 ng/ml which is comparable to that for the original extraction procedure. Within-batch precision over the range of 9-90 ng/ml averages 5.8% and at the lower limit of detection 17.3%. Between-batch precision over the range of 9-90 ng/ml averages 8.5% and at the lower limit of detection 19.7%. No significant difference could be found in the quantitative determination of verapamil in 27 plasma samples from patients undergoing verapamil treatment using the original and modified extraction procedures. This improvement of the extraction procedure simplifies the determination of verapamil and the only additional material required is liquid N2 or a suitable solvent cooled with dry ice.

This publication has 1 reference indexed in Scilit: