HPLC Separation and FT-IR Isomer Differentiation of the 1,2,4,7/1,2,4,8-Tetrachlorodibenzodioxin Isomer Pair—A Theoretical/Empirical Approach to Dibenzodioxin Isomer Assignment
- 1 February 1988
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 42 (2) , 321-326
- https://doi.org/10.1366/0003702884428383
Abstract
The 1,2,4,7- and 1,2,4,8-tetrachlorodibenzodioxin (TCDD) isomers were separated by reversed-phase high-performance liquid chromatography (HPLC) with the use of a pyrene column. Fourier transform infrared (FT-IR) matrix isolation and vapor-phase spectra of the individual isomers were recorded. The spectra of the HPLC-separated isomers correlate well with spectral subtraction results and were found to be distinct in three spectral regions—one of which allows for isomer structural assignment. Ambiguities and differences in published TCDD isomer FT-IR assignments are discussed in terms of a qualitative valence-bond approach and empirically derived estimates of ether linkage asymmetric stretching frequencies.This publication has 5 references indexed in Scilit:
- Tetrachlorodibenzo-p-Dioxin Isomer Differentiation by Capillary Gas Chromatography Fourier Transform-Infrared SpectroscopyApplied Spectroscopy, 1987
- Micro-Diffuse Reflectance and Matrix Isolation Fourier Transform Infrared Techniques for the Identification of TetrachlorodibenzodioxinsApplied Spectroscopy, 1986
- Tetrachlorodibenzodioxin isomer differentiation by micro diffuse reflectance Fourier transform infrared spectrometry at the low nanogram levelAnalytical Chemistry, 1985
- Identification of polychlorinated dibenzo-p-dioxin isomers by powder x-ray diffraction with electron capture gas chromatographyAnalytical Chemistry, 1983
- Synthesis of new steric and electronic analogs of 2,3,7,8-tetrachlorodibenzo-p-dioxin.Environmental Health Perspectives, 1973