Mutations in lambda repressor's amino-terminal domain: implications for protein stability and DNA binding.

Publisher Website
Google Scholar
Abstract
The DNA binding properties of 52 different single-amino acid substitutions in .lambda. repressor''s amino-terminal domain were characterized. Seven proteins bearing mutations that change solvent-exposed side chains were purified. The amino-terminal domains of these mutant repressors are folded and are comparable to the wild-type amino-terminal domain in thermal stability. A purified mutant repressor bearing a substitution in a buried side chain contains an amino-terminal domain with decreased thermal stability. Mutations that alter solvent-exposed wild-type side chains may define residues that form the operator DNA binding surface of .lambda. repressor whereas completely or partially buried mutations exert their effect by decreasing protein stability.