Synergism of Diflubenzuron and Teflubenzuron in Larvae of Beet Armyworm (Lepidoptera: Noctuidae)

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Abstract
Spodoptera exigua (Hübner), a serious lepidopterous pest in greenhouse crops in the Netherlands, is rather insensitive to the chitin synthesis inhibitor dHlubenzuron. The LC50 for fourth and fifth instars treated with diflubenzuron were 295 and 16 mg/liter, respectively; for teflubenzuron, LC50’s were 42 and 7 mg/liter, respectively. To optimize the use of benzoylphenyl urea in S. exigua larvae, a combination with four synergists was studied. The best results were obtained with profenofos and S,S,S-tributylphosphorotrithioate (DEF), both of which are hydrolase inhibitors. These synergists enhanced the toxicity of diflubenzuron in fourth instars nine and six fold, respectively. Diethylmaleate, a glutathion S-transferase inhibitor, decreased the LC50 five fold, whereas piperonyl butoxide, an oxidase inhibitor, was responsible for only a three-fold reduction.

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