Changes in the Virulence of Naegleria fowleri Maintained In vitro

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Abstract
After a prolonged period of maintenance in Nelson''s axenic medium, the HB-1 and C-66 strains of pathogenic N. fowleri lost their original virulence for mice. Cloned low virulence substrains were established and compared to cloned high virulence substrains that were maintained on monkey cell (MKC) cultures. Some differences in physiology between the low virulence and high virulence substrains were shown by their growth in Nelson''s medium and MKC cultures, cytopathic effects, catalase production, as well as in pathogenicity for mice inoculated intranasally. The importance of the identification of the factors which may contribute to the loss or acquisition of virulence of these free-living amebae is stressed.

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