Assembly of Staphylococcus aureusγ‐hemolysin into a pore‐forming ring‐shaped complex on the surface of human erythrocytes
- 30 June 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 410 (2-3) , 333-337
- https://doi.org/10.1016/s0014-5793(97)00618-2
Abstract
Staphylococcal γ‐hemolysin consists of Hlg1 (or Luk F) of 34 kDa and Hlg2 of 32 kDa, which cooperatively lyse human erythrocytes. Since γ‐hemolysin caused swelling of human erythrocytes prior to lysis, we studied pore‐forming nature of the toxin by use of polyethylene glycols as osmotic protectants and determined the functional diameter of the pore. To elucidate the molecular architecture of the membrane pore formed by γ‐hemolysin, we solubilized the pore complex with 2% sodium dodecyl sulfate, separated it from erythrocyte membrane proteins by sucrose gradient ultracentrifugation, and observed the isolated complex under an electron microscope. Our data showed that Hlg1 and Hlg2 of γ‐hemolysin assemble into a ring‐shaped 195 kDa complex in a molar ratio of 1 : 1, which may form a membrane pore with a functional diameter of 2.1–2.4 nm.Keywords
This publication has 18 references indexed in Scilit:
- Sequential Binding of Staphylococcal y-Hemolysin to Human Erythrocytes and Complex Formation of the Hemolysin on the Cell Surface†Bioscience, Biotechnology, and Biochemistry, 1997
- Essential Binding of LukF of Staphylococcalγ-Hemolysin Followed by the Binding of HγII for the Hemolysis of Human ErythrocytesBioscience, Biotechnology, and Biochemistry, 1995
- The two Staphylococcal bi‐component toxins, leukocidin and gamma‐hemolysin, share one component in commonFEBS Letters, 1993
- Improved Method for Purification of Leukocidin and Gamma-Hemolysin Components fromStaphylococcus aureusBioscience, Biotechnology, and Biochemistry, 1993
- Gamma-Hemolysin Genes in the Same Family withLukFandlukSGenes in Methicillin ResistantStaphylococcus aureusBioscience, Biotechnology, and Biochemistry, 1993
- Molecular cloning and nucleotide sequence of leukocidin F-component gene (lukF) from methicillin resistant Staphylococcus aureusBiochemical and Biophysical Research Communications, 1992
- Nucleotide sequence of leukocidin S-component gene (lukS) from methicillin resistant Staphylococcus aureusBiochemical and Biophysical Research Communications, 1991
- Ionic and osmotic equilibria of human red blood cells treated with nystatin.The Journal of general physiology, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Staphylococcus γ hæmolysinThe Journal of Pathology and Bacteriology, 1938