In Vitro Propagation of Carrizo Citrange1

Abstract
In vitro proliferation of shoot tips was attempted with nucellar seedlings of sour orange (Citrus aurantium L.), Carrizo citrange (‘Washington’ navel × Poncirus trifoliata (L.) Raf.), and Cleopatra mandarin (C. reshni Hort. ex Tanaka). Shoot multiplication was achieved with Carrizo citrange shoot tips cultured on Knop’s medium (with organics) supplemented with Murashige and Skoog microelements, 5 mg/liter 6-benzyIamino purine (BA), 3 — 4% sucrose and at a light intensity of 2.2 klx. No effect on shoot proliferation was obtained with 6-furfurylamino purine (kinetin), 6 γ-γ-dimethallyl amino purine (2iP), agar concentration, addition of orange juice, or nitrogen source. Proliferated shoot tips of Carrizo citrange rooted when cultured on Murashige and Tucker’s medium containing 1 mg/liter napthaleneacetic acid and gelled with 0.5% agar. Plants were successfully established in soil.

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