PHOTON-COUNTING IMAGING - APPLICATIONS IN BIOMEDICAL-RESEARCH

  • 1 March 1989
    • journal article
    • research article
    • Vol. 7  (3) , 262-+
Abstract
Photon counting imaging, a technique capable of imaging at the single photon level, is finding applications in biological research and is providing unprecedented views of ultra-low light level phenomena. In combination with the optical microscope, the technique has provided a means of directly visualizing gene expression in single cells, imaging metabolites in tumor tissue and visualizing the chemiluminescence associated with oxidative metabolism in phagocytic cells. At the macroscopic level, it has greatly extended the sensitivity of detection in protein blots and has been applied as an image luminometer to assay microtiter plates. The technique holds great promise for use with fluorscence- luminescence-based methods in many fields of research.