Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides
- 1 September 1994
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 5 (9) , 867-869
- https://doi.org/10.1016/1044-0305(94)87010-1
Abstract
The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8∶1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20∶1 for a solution containing 32 attomoles/μL.Keywords
This publication has 1 reference indexed in Scilit:
- Micro-electrospray mass spectrometry: Ultra-high-sensitivity analysis of peptides and proteinsJournal of the American Society for Mass Spectrometry, 1994