Abstract
Rhizopus nigricans grows in a synthetic medium containing pyruvic acid as the only source of C, with formation of fumaric acid at about 32% of the theoretical yield; acetic and lactic acids are produced simultaneously. Pyruvic acid would thus appear to be a probable intermediate stage in formation of fumaric acid from sugar by Rhizopus, acetaldehyde, acetic acid, and succinic acid being postulated as intermediate stages between pyruvic and fumaric acids.

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