Some Limitations of Using Equilibrium Dialysis to Study Human Serum Albumin-Testosterone Interaction*
- 1 March 1978
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 46 (3) , 501-503
- https://doi.org/10.1210/jcem-46-3-501
Abstract
The apparent association constant of testosterone binding to pure human serum albumin was found to be significantly less at 5 g/dl (1.79 X 104 M-1) than at 1 g/dl (2.29 X 104 M-1). This demonstrates that the common assumption that binding site characteristics are constant with serum protein dilution is not strictly valid. Determination of the percent free testosterone at normal serum protein concentrations by equilibrium dialysis may also be in error by 20% or more due to fluid shifts within the test system.Keywords
This publication has 4 references indexed in Scilit:
- Stability of testosterone and androstenedione in blood and plasma samplesClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- Studies on the binding of testosterone to human plasmaSteroids, 1968
- Steroid-Protein Interaction with Particular Reference to Testosterone Binding by Human SerumJournal of Biological Chemistry, 1967
- BINDING OF THYROXINE BY SERUM PROTEINS EVALUATED BY EQUILIBRIUM DIALYSIS AND ELECTROPHORETIC TECHNIQUES. ALTERATIONS IN NON-THYROIDAL ILLNESS*Journal of Clinical Investigation, 1963