Structural study of translating 70 S ribosomes from Escherichia coli
- 2 May 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 155 (1) , 167-172
- https://doi.org/10.1016/0014-5793(83)80232-4
Abstract
Translating 70 S ribosomes of Escherichia coli either in the pre‐translocation or in the post‐translocation state have been prepared by using the cell‐free translation system in poly(U)—S—S—Sepharose columns [Methods Enzymol. (1979) 59, 382–398]. Electron microscopy study of the preparations has demonstrated that: (1) the mutual orientation of the ribosomal subunits in the translating ribosomes is the same as proposed by Lake for routine 30 S·50 S couples [J. Mol. Biol. (1976) 105, 111–130]; (2) the L7/L12 stalk of the 50 S subunit sticks out from the 70 S particle and does not join the 30 S subunit; (3) pre‐translocation and post‐translocation state ribosomes do not differ in mutual orientation of the subunits and in the position of the L7/L12 stalk, within the limits of electron microscopy resolution.Keywords
This publication has 10 references indexed in Scilit:
- Arrangement of the subunits in the ribosome of Escherichia coli: demonstration by immunoelectron microscopy.Proceedings of the National Academy of Sciences, 1981
- Mutants of Escherichia coli lacking ribosomal protein L1Journal of Molecular Biology, 1981
- Ribosome structure: Hand determination by electron microscopy of 30 S subunitsJournal of Molecular Biology, 1979
- [30] The use of columns with matrix-bound polyuridylic acid for isolation of translating ribosomesPublished by Elsevier ,1979
- Ribosomal proteins localized at a single region of the large subunit by immune electron microscopyJournal of Molecular Biology, 1978
- Ribosome structure determined by electron microscopy of Escherichia coli small subunits, large subunits and monomeric ribosomesJournal of Molecular Biology, 1976
- Structural dynamics of bacterial ribosomesJournal of Molecular Biology, 1976
- Architecture of the Escherichia coli ribosome as determined by immune electron microscopy.Proceedings of the National Academy of Sciences, 1975
- Isolation of translating ribosomes with a resin-bound poly-U columnFEBS Letters, 1975
- Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coliBiochemistry, 1968