A Simple Method for Collecting Infective Larvae of Litomosoides carinii

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Abstract
A method is described whereby substantial quantities of infective larvae of L. carinii can be obtained with relative ease. In each of 2 separate experiments, > 5000 infective larvae were recovered by soaking the pelts of 5 or 7 Mongolian jirds (Meriones unguiculatus) exposed for 6 or 7 h to infected tropical rat mites (Ornithonyssus bacoti). The mites were infected 7-8 or 9-10 days earlier by exposure to jirds with patent infections. Of the total number of infective larvae, 94% recovered and migrated out of the pelts within 2 1/2 h. Jirds inoculated s.c. with infective larvae collected in this manner exhibited high mean percent recoveries at necropsy, i.e., 79 and 60 at 5 and 29 days postinoculation, respectively. The ranges in worm recoveries were narrow.

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