Effects of blood-free and protein-free perfusion on CFC in the isolated cat hindlimb

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Abstract
To investigate the effects of blood and protein on capillary filtration coefficient (CFC), isolated cat hindlimbs were perfused at constant flow from a reservoir containing one of three perfusates: 1) an electrolyte solution also used for dialyzing bovine serum albumin (BSA) and hence labeled dialysate, 2) dialyzed 6.5–7.0 g/100 ml BSA, labeled albumin, and 3) albumin plus cat blood, labeled control. CFC during control perfusion averaged 0.0137 +/- 0.003 (SD, n = 26) and increased slowly with time. CFCs with dialysate and albumin were 3.1 +/- 0.56 (n = 11) and 1.7 +/- 0.52 (n = 15) times control, respectively. However, the ratio in a particular experiment depended on control CFC, the ratio being low when the control CFC was low and high when control CFC was high. The data can be explained if water flow occurs through two types of transcapillary pathways, one having a hydraulic capacity constant from animal to animal and insensitive to blood and protein, the second varying in hydraulic capacity and requiring blood and protein for its normal values.

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