Stabilization of xylanase by random mutagenesis
- 25 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 316 (2) , 123-127
- https://doi.org/10.1016/0014-5793(93)81199-a
Abstract
Four heat‐resistant mutants of xylanase (N56, N102, N104 and F1) were obtained by random mutagenesis. The mutant genes had the following amino acid changes: N56, Ser‐26 to Trp, Gly‐38 to Asp and Thr‐126 to Ser; N102, Gly‐38 to Asp; N104, Gly‐38 to Ser and Arg‐48 to Lys; F1, Ser‐12 to Cys. Kinetic studies showed that N104 is stabilized by an increase in the activation enthalpy, while the other mutants are stabilized by a decrease in the activation entropy.Keywords
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