The human sperm protein PH‐20 has hyaluronidase activity
- 28 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (3) , 545-548
- https://doi.org/10.1016/0014-5793(93)80873-s
Abstract
The PH‐20 protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [(1991) J. Cell Biol. 111, 2939‐2949]. We have isolated the cDNA encoding the human PH‐20 protein from a testis library. This cDNA was expressed in RK 13 cells using a vaccinia virus expression system. Cells expressing the human PH‐20 protein possess hyaluronidase activity. Treatment with PI‐PLC releases the hyaluronidase into the the medium with a concomitant large increase in enzymatic activity. These results demonstrate that the human PH‐20 protein has hyaluronidase activity.Keywords
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